Expression and functional role of LncRNA GSEC in oral squamous cell carcinoma
DOI:
https://doi.org/10.24079/CAJMS.2025.04.001Keywords:
Proliferation, Apoptosis, OSCC, LncRNA GSECAbstract
Objective: The current investigation seeks to elucidate the regulatory impact of the long non-coding RNA GSEC (lncRNA GSEC) on oncogenic mechanisms in oral squamous cell carcinoma. Methods: The expression dynamics of GSEC were systematically mapped across in vitro systems, including healthy oral keratinocyte controls (NHOK) and their malignant counterparts (SCC-25, SCC-9, CAL-27), utilizing standardized qRT-PCR protocols. CAL-27 (highest GSEC expression) underwent siRNA-mediated knockdown. The assessment of cellular functions such as proliferation, apoptosis regulation, mobility, and invasion was conducted utilizing validated protocols: CCK-8 for cell viability assessment, flow cytometry for apoptotic cell identification, Transwell systems for measuring invasive ability, and scratch assay for migration analysis. mRNA and protein levels of proliferation/apoptosis markers (Ki67, PCNA, BCL-2, BAX, caspase-9) were analyzed by qRT-PCR, Western blot, and immunofluorescence. Results: GSEC was significantly upregulated in OSCC cells vs. NHOK (p<0.05), peaking in CAL-27 (p<0.05 vs. SCC-9/SCC-25). Downregulation of GSEC inhibited the proliferation, migration, and invasive capabilities of OSCC cells and promoted apoptosis with statistical significance (p<0.05). A decrease in Ki67 and PCNA expression, accompanied by an increase in BAX and caspase-9 and a decrease in BCL-2 levels, was detected at the mRNA and protein levels, with all reaching statistical significance at the p<0.05 threshold. Conclusion: GSEC overexpression promotes OSCC malignancy by driving proliferation and inhibiting apoptosis. Targeting GSEC may offer diagnostic and therapeutic potential for OSCC.
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