Dehydrocostus Lactone Down - regulates Lipopolysaccharide-induced SAA3 gene activation in the Microglia.

Authors

  • Zhao Fuquan Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Seesregdorj Surenjid Department of Mongolian Medicine Study, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Bilegtsaikhan Tsolmon Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Oyunchimeg Erdenee Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Tsevelmaa Nanjidsuren Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Uurtuya Shuumarjav Department of Medical Law and Education Study, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Shiirevnyamba Avirmed Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia https://orcid.org/0000-0002-1010-8221

DOI:

https://doi.org/10.24079/CAJMS.2023.12.006

Keywords:

Microglia, Dehydrocostus Lactone, Lipopolysaccharide, Serum amyloid A, SAA3 gene

Abstract

Running Title: DDL inhibits the LPS-induced SAA3 gene expression in microglia
Objective: Research on the mechanism of Dehydrocostus lactone (DDL) in microglial cells of the brain needs to be improved, despite its reported anti-inflammatory, anticancer, and anti-proliferation properties as a natural product of Eerdun Wurile The precise functions of the Serum amyloid A (SAA) genes are not yet defined. However, these genes are suggested to possess antibacterial properties and can attract monocytes and neutrophils. On the other hand, SAA genes can also induce inflammatory cytokines and metalloproteinases in smooth muscle cells and macrophages, thus promoting inflammation. This study aimed to investigate the impact of DDL on SAA3 gene expression in microglial cells stimulated by LPS.
Methods: The anti-inflammatory effects of DDL were studied using LPS-stimulated murine BV2 microglia. BV2 was cultured in DMEM, and then 4µM DDL was added. Then BV2 was treated with one ng/ml LPS for 24 hours to stimulate.
Results: LPS treatment increased the expression of SAA3 mRNA in BV2 microglial cells, while DDL pre-treatment inhibited LPS-induced SAA3 mRNA expression.
Conclusion: Microglia cells treated with LPS display a significant reduction in the expression of SAA3 gene transcripts upon treatment with DDL.

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Author Biographies

Zhao Fuquan, Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Department of Mongolian Medicine Study, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia;

Department of Neuropathology, Hospital of Inner Mongolian University for the Nationalities, Tongliao, China;

Oyunchimeg Erdenee, Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Division of Education and Research, National Center for Communicable Disease, Ulaanbaatar, Mongolia

Tsevelmaa Nanjidsuren, Department of Health Research, Graduate School, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Institute of Bio-Medical Sciences, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

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Published

2023-12-16

How to Cite

Fuquan, Z., Surenjid, S., Tsolmon, B., Erdenee, O., Nanjidsuren, T., Shuumarjav, U., & Avirmed, S. (2023). Dehydrocostus Lactone Down - regulates Lipopolysaccharide-induced SAA3 gene activation in the Microglia. Central Asian Journal of Medical Sciences, 9(4), 195–200. https://doi.org/10.24079/CAJMS.2023.12.006

Issue

Vol. 9 No. 4 (2023)

Section

Case Reports

License

Copyright (c) 2023 Mongolian National University of Medical Sciences

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