Regulating Action of in Vitro Hepatitis C Virus Infection on Interferon-Induced Interferon Stimulating Genes in Murine Macrophages

Abstract

Objectives: The objective of this study was to determine the in vitro regulating effect of Hepatitis C virus (HCV) infection on interferon stimulating genes (ISGs) stimulated by IFN-ү and Imiquimod (TLR7 ligand) on murine macrophages. iNOS and STAT1 were measured by RT-PCR, and immunoblotting (IB). Nitric oxide production was measured by Griess Reagent Assay. Objectives: The objective of this study was to determine the in vitro regulating effect of Hepatitis C virus (HCV) infection on interferon stimulating genes (ISGs) stimulated by IFN-ү and Imiquimod (TLR7 ligand) on murine macrophages. iNOS and STAT1 were measured by RT-PCR, and immunoblotting (IB). Nitric oxide production was measured by Griess Reagent Assay. Results: HCV inhibits IFN induced iNOS mRNA and also protein expression. HCV significantly reduced IFN-ү induced ISGs (iNOS mRNA, iNOS protein, s727-STAT1, tyr701- STAT1). Conclusion: These results indicate that in vitro hepatitis C virus infection is involved in the regulation of IFN-ү induced ISGs in the levels of gene and protein expression of iNOS and STAT1 transcription factors.: HCV inhibits IFN induced iNOS mRNA and also protein expression. HCV significantly reduced IFN-ү induced ISGs (iNOS mRNA, iNOS protein, s727-STAT1, tyr701- STAT1). Conclusion: These results indicate that in vitro hepatitis C virus infection is involved in the regulation of IFN-ү induced ISGs in the levels of gene and protein expression of iNOS and STAT1 transcription factors.