The Potential Effect of Traditional Herbal Medicine on Bile Flow in CC14-Induced Liver Injury Model
DOI:
https://doi.org/10.24079/cajms.2024.03.004Keywords:
Carbon, Organiz Chemical, Liver Disease, Drug Induced Liver Injury, Bile AcidAbstract
Objective: To investigate whether the modified Mongolian traditional medicine Yaman Serdeg-3 (NYS-3) effects the biliary excretion in the carbon tetrachloride-induced liver injury model. Methods: Forty-seven male and female Sprague-Dawley rats were divided into 5 groups: Group I healthy group (n=12); Group II healthy group + NYS-3 (n=9); Group III liver injury model (n=10); Group IV liver injury model + Chinese medicine Hua gan Pian (n=10); Group V liver injury model + NYS-3. Experimental groups II-V, received corresponding medicinal preparations for 2 weeks. The dose of Hua gan Pian (HGP) was 0.046g/ml while the dose of NYS-3 was 2.025g/ml. For the acute induction of liver injury, the rats were orally administered 40% CCl4 in olive oil (4 ml/kg) twelve hours after the last administration of preparations. Results: At the first 15-minute interval, the highest bile flow was observed in Group II rats, which were administered NYS-3. This phenomenon continued at the second 15-minute interval, also. However, at the third 15-minute interval, the bile flow decreased sharply in Group II. On the other hand, in the liver injury model, untreated or pre-treated rats had significantly different bile flows. For example, in the first half of the experiment, both non-treated and HGP pre-treated groups had similar bile flow, while, the group pre-treated with NYS-3 had significantly low bile flow. Then, after 45 minutes of the experiment, the bile flow became similar amounts in both pre-treated groups. Conclusion: We conclude that the NYS-3 administration had increased the bile flow at 3rd 15min of interval, which is higher than positive control group where liver injury model rats received HGP.
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