Determination of Antimicrobial Activity of Antibiotics Encapsulated in Liposomes

Authors

  • Enkhtaivan Erdene Department of Microbiology, Infection Prevention and Control, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Enkhjargal Dorjbal School of Pharmacy, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Oidov Baatarkhuu Department of Infectious Diseases, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia https://orcid.org/0000-0002-0769-3693
  • Ariunsanaa Byambaa Department of Microbiology, Infection Prevention and Control, School of Biomedicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia. https://orcid.org/0000-0001-8974-2892

DOI:

https://doi.org/10.24079/cajms.2024.01.002

Keywords:

Phospholipids, Liposome, Antibiotic, Nanoparticle, MRSA

Abstract

Objective: Antibiotic-resistant infections caused by bacteria are already a serious concern for humanity, and by 2050, they are most likely to overtake all other causes of death. One of the most frequent causes of skin infections is methicillin-resistant Staphylococcus aureus (MRSA), whose resistance to most medications makes treatment challenging. This study aimed to determine the antibacterial activity of liposome-encapsulated doxycycline compared to free doxycycline.
Methods: This study was conducted using an experimental design. Our study isolated and purified the phospholipid (PL) fraction from egg yolk. Antibiotics were encapsulated using the freeze-thaw process, and phospholipids were extracted via intermittent evaporation. Finally, the liposome's minimum inhibitory concentration (MIC) was effective against MRSA.
Results: Phospholipid fraction isolated from egg yolks with 32% extraction yield of phosphatidylcholine. The thin phase chromatography fraction was phospholipids with a retention factor (Rf) of 0.39. The effectiveness of liposomal doxycycline hyclate over free doxycycline was demonstrated by in vitro tests.
Conclusion: The results showed that a liposome containing phosphatidylcholine could be a tremendous topical antimicrobial construct for treating MRSA infections.

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Author Biography

Oidov Baatarkhuu, Department of Infectious Diseases, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Co-Corresponding Author

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Published

2024-03-15

How to Cite

Erdene, E., Dorjbal, E., Baatarkhuu, O., & Byambaa, A. (2024). Determination of Antimicrobial Activity of Antibiotics Encapsulated in Liposomes. Central Asian Journal of Medical Sciences, 10(1), 8–16. https://doi.org/10.24079/cajms.2024.01.002

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Articles