Effects of Mongolian Medicine Sengdeng-4 in Rabbit Knee Osteoarthritis Model

Authors

  • Jingang International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Qimunge International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Hugejiletu International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Hasitana Second Hospital of Baotou City, Inner Mongolia Autonomous Region, People’s Republic of China
  • Yanhua Xu
  • Baigalmaa Jigden International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Bold Sharavyn International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia;

DOI:

https://doi.org/10.24079/cajms.2023.06.006

Keywords:

Mongolian medicine, Seng ldeng-4, Knee osteoarthritis, Inflammation

Abstract

Objectives: Seng ldeng-4(SD-4) has a long history of use in Mongolian medical clinics for the treatment for rheumatic disorders. The objective of this study was to investigate the therapeutic effect and mechanism of SD-4 on rabbits with knee osteoarthritis induced by Hulth’s method.
Methods: SD-4 (0.428and 0.857g·kg-1) or Zhuangguguanjie pill (0.514g·kg-1) were orally administered in rabbits for four weeks after the induction of knee osteoarthritis using Hulth’s method. Inflammation factors (TNF-α, IL-1β, and NO) and histopathological lesions in the knee joint were examined by ELISA and HE is staining. The expression of type II collagen mRNA in the articular cartilage was detected using real-time qPCR.
Results: SD-4 ameliorated the articular lesion damage caused by Hulth’s method in rabbits and reduced the serum levels of inflammatory cytokines TNF-α, IL-1β, and NO. The qPCR results showed that the mRNA expression of type II collagen (COL2) and miRNA-140 gene in the model group was significantly lower than that in the control group (P<0.01), while SD-4 treatment significantly elevated their expressions in the articular cartilage.
Conclusion: SD-4 can improve articular cartilage morphology and inflammation by regulating miRNA-140 and COL2 gene expressions, and thus ameliorating knee osteoarthritis of rabbits induced by Hulth’s method.

Abstract
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Author Biographies

Jingang, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia Autonomous Region, People’s Republic of China

Qimunge, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia Autonomous Region, People’s Republic of China

Yanhua Xu

Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia Autonomous Region, People’s Republic of China

References

Fu B, Bao LY. Progress in research on osteoarthritis. Journal of Liaoning University of Traditional Chinese Medicine. 2014; 16(7): 243-245.

Gui YS. Arthritis summary. Beijing, China: China Medical Science and Technology Press; 2004, p 58-59.

Ba GN, Tu YX. Mongolian medicine formula. Hohhot, China: Inner Mongolia People Publishing House; 2007, p 148.

Xu L. Basic research on the efficacy of Mongolian medicine Senden-4/Doctor's thesis, Shenyang, China: Shenyang Pharmaceutical University; 2007.

Zhi GL, Mu D. Mongolian medicine treatment of joint yellow water disease experience. Chinese Journal of Medicine. 2015; 4:22.

Du R, Zhao P, Wu S, et al. Sendeng-4 suppressed melanoma growth by induction of autophagy and apoptosis. Evid Based Complement Alternat Med. 2021;5519973. https://doi.org/10.1155/2021/5519973 PMid:34475961 PMCid:PMC8407990

Zhang Y, Du R, Zhao P, et al. Preparation, and characterization of natural quercetin-based Mongolia medicine Sendeng-4 nano emulsion (N-QUE-NE) and its Antibacterial Activity. Curr Drug Deliv. 2021;18(7):984-993. https://doi.org/10.2174/1567201818666201214143631 PMid:33319681

Wang X, Li D, Jiang M, Pei Z, Xu L. K nearest neighbor algorithm coupled with metabonomic to study the therapeutic mechanism of Sendeng-4 in adjuvant-induced rheumatoid arthritis rat. Evid Based Complement Alternat Med. 2018;2484912. https://doi.org/10.1155/2018/2484912 PMid:29681970 PMCid:PMC5842719

Chen GY, Chen JQ, Liu XY, et al. Total flavonoids of rhizoma drynariae restore the MMP/TIMP balance in models of osteoarthritis by inhibiting the activation of the NF-κB and PI3K/AKT pathways. Evid Based Complement Alternat Med. 202; 6634837. https://doi.org/10.1155/2021/6634837 PMid:33995548 PMCid:PMC8081598

Khasag A, Byambasukh O, Narmandakh B, Badarch A, Shukhert T, Unurjargal T. The efficacy and safety of "Kyushin" in Mongolian patients with ischemic heart disease and heart failure. Cent Asian J Med Sci. 2020;6:249-255. https://doi.org/10.24079/cajms.2020.12.006

Zhang HDBLG, Wang XL, Enkhsuren E, Tu BRB, Dorjbal E, Damdinjav D. Mongolian medicine Honghu-Qiqige ethanol extract exhibits anti-inflammation role in arthritis. Cent Asian J Med Sci. 2021;7:170-177. https://doi.org/10.24079/cajms.2021.06.013

Tu HBSHL, Tumurbaatar K, Chimedtseren C, Bukhbayar T, Damba T. Results of the study for the antihypertensive effect in the Panzeria Alaschanica Kupr plant. Cent Asian J Med Sci. 2022; 8:102-111. https://doi.org/10.24079/cajms.2022.06.004

Duan DB, Zhang SY. Effect of sodium hyaluronate combined with celecoxib on osteoarthritis and its effect on TNF-α, IL-1β and PGE2. Med. Rev. 2016, 22(23): 4729-4732.

Gu HL, Liu YL, Wang WL. Leptin on IL-1β induces rat osteoarthritis chondrocytes. J. Southeast Univ. Nat. Sci .Ed (Medical Edition). 2015; 34(06): 870-875.

Yang DF, Ye W, Wang XG. Study on interleukin-1β on matrix metalloproteinase-3, 7, 9, 13- 46 - in nucleus pulposus cells. Chinese Journal of Osteoporosis. 2007; 13(8): 559-563.

Qiu QH, Li Q, Li J. Experimental study on the effects of new fire on NO and IL-1 in rabbit knee osteoarthritis. New Chinese Medicine Journal. 2013; 45(10): 133-135.

Wang F, Liu G, Shi J, Wang J, Zheng S, Shi Q, Pan H. Effects of glucosamine hydrochloride combined with non-steroidal anti-inflammatory drugs on symptoms and HSS scores in patients with knee osteoarthritis. Pak J Pharm Sci. 2023;36(4(Special):1337-1341.

Hutcherson CW, Mao M, Thakur B, Dhaher YY. Low-grade inflammatory mediators and metalloproteinases yield synchronous and delayed responses to mechanical joint loading. Cartilage. 2023; 19476035231193089. https://doi.org/10.1177/19476035231193089 PMid:37614184

Lee H, Nam J, Jang H, Park YS, Son MH, Lee IH, Eyun SI, Jeon J, Yang S. Novel molecule BBC0901 inhibits BRD4 and acts as a catabolic regulator in the pathogenesis of osteoarthritis. Biomed Pharmacother. 2023; 166:115426. https://doi.org/10.1016/j.biopha.2023.115426 PMid:37666177

Chen L, Yang XL. Expression of inflammatory factors IL-1β, IL-6 and COX-2 in serum of patients with osteoarthritis. Chinese Clinical Medicine. 2016;23(1):61-62.

Li Q, Xu JY, Hu X, Li J, Huang XJ, Wu ZY, Wang DG, Ge YB. The protective effects and mechanism of Ruyi Zhenbao Pill, a Tibetan medicinal compound, in a rat model of osteoarthritis. J Ethnopharmacol. 2023;308:116255. https://doi.org/10.1016/j.jep.2023.116255 PMid:36809823

Rabie MA, Sayed RH, Venkatesan JK, Madry H, Cucchiarini M, El Sayed NS. Intra-articular injection of rAAV-hFGF-2 ameliorates monosodium iodoacetate-induced osteoarthritis in rats via inhibiting TLR-4 signaling and activating TIMP-1. Toxicol Appl Pharmacol. 2023; 459:116361. https://doi.org/10.1016/j.taap.2022.116361 PMid:36584762

Zhang P, Dong B, Yuan P, Li X. Human umbilical cord mesenchymal stem cells promoting knee joint chondrogenesis for the treatment of knee osteoarthritis: a systematic review. J Orthop Surg Res. 2023;18(1):639. https://doi.org/10.1186/s13018-023-04131-7 PMid:37644595 PMCid:PMC10466768

Deng Y, Zhang M, Li W, et al. Investigation of the therapeutic effect and mechanism of holographic meridian scraping therapy on knee osteoarthritis. Altern Ther Health Med. 2023; AT8727.

Yao N, Chen GC, Lu YY, et al. Bushen Qiangjin capsule inhibits the Wnt/α-catenin pathway to ameliorate papain-induced knee osteoarthritis in rat. J Tradit Chin Med. 2021;41(6):935-942. doi: 10.19852/j.cnki.jtcm.2021.06.010

Lin W, Xie L, Zhou L, Zheng J, Zhai W, Lin D. Effects of platelet-rich plasma on subchondral bone marrow edema and biomarkers in synovial fluid of knee osteoarthritis. Knee. 2023;42:161-169. https://doi.org/10.1016/j.knee.2023.03.002 PMid:37001332

Lohberger B, Kaltenegger H, Eck N, et al. Derivatives Inhibit Inflammation Processes and Modulate MAPK Signaling in Human Healthy and Osteoarthritis Chondrocytes. Int J Mol Sci. 2022;23(6):3396. https://doi.org/10.3390/ijms23063396 PMid:35328817 PMCid:PMC8955849

Lin TC, Lin JN, Yang IH, et al. The combination of resveratrol and Bletilla striata polysaccharide decreases inflammatory markers of early osteoarthritis knee and the preliminary results on LPS-induced OA rats. Bioeng Transl Med. 2022;8(4): e10431. https://doi.org/10.1002/btm2.10431 PMid:37476067 PMCid:PMC10354757

Katoh S, Yoshioka H, Senthilkumar R, Preethy S, Abraham SJK. Enhanced miRNA-140 expression of osteoarthritis-affected human chondrocytes cultured in a polymer based three-dimensional (3D) matrix. Life Sci. 2021; 278:119553. https://doi.org/10.1016/j.lfs.2021.119553 PMid:33932445

Si HB, Zeng Y, Liu SY, et al. Intra-articular injection of microRNA-140 alleviates osteoarthritis progression by modulating extracellular matrix homeostasis in rats. Osteoarthritis Cartilage. 2017;25(10):1698-1707. https://doi.org/10.1128/MCB.05178-11 PMid:21576357 PMCid:PMC3133397

Nakamura Y, Wang GX, Li Z, et al. Chondrocyte-specific microRNA-140 regulates endochondral bone development and targets Dnpep to modulate morphogenetic protein signaling. Mol cell Biol. 2011;31: 3019-3028. https://doi.org/10.1128/MCB.05178-11 PMid:21576357 PMCid:PMC3133397

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Published

2023-06-30

How to Cite

Jingang, Qimunge, Hugejiletu, Hasitana, Xu, Y., Jigden, B., & Sharavyn, B. (2023). Effects of Mongolian Medicine Sengdeng-4 in Rabbit Knee Osteoarthritis Model. Central Asian Journal of Medical Sciences, 9(2), 42–49. https://doi.org/10.24079/cajms.2023.06.006

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