Effects of Mongolian Medicine Sengdeng-4 in Rabbit Knee Osteoarthritis Model

Authors

  • Jingang International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Qimunge International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Hugejiletu International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Hasitana Second Hospital of Baotou City, Inner Mongolia Autonomous Region, People’s Republic of China
  • Yanhua Xu
  • Baigalmaa Jigden International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Bold Sharavyn International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia;

DOI:

https://doi.org/10.24079/cajms.2023.06.006

Keywords:

Mongolian medicine, Seng ldeng-4, Knee osteoarthritis, Inflammation

Abstract

Objectives: Seng ldeng-4(SD-4) has a long history of use in Mongolian medical clinics for the treatment for rheumatic disorders. The objective of this study was to investigate the therapeutic effect and mechanism of SD-4 on rabbits with knee osteoarthritis induced by Hulth’s method.
Methods: SD-4 (0.428and 0.857g·kg-1) or Zhuangguguanjie pill (0.514g·kg-1) were orally administered in rabbits for four weeks after the induction of knee osteoarthritis using Hulth’s method. Inflammation factors (TNF-α, IL-1β, and NO) and histopathological lesions in the knee joint were examined by ELISA and HE is staining. The expression of type II collagen mRNA in the articular cartilage was detected using real-time qPCR.
Results: SD-4 ameliorated the articular lesion damage caused by Hulth’s method in rabbits and reduced the serum levels of inflammatory cytokines TNF-α, IL-1β, and NO. The qPCR results showed that the mRNA expression of type II collagen (COL2) and miRNA-140 gene in the model group was significantly lower than that in the control group (P<0.01), while SD-4 treatment significantly elevated their expressions in the articular cartilage.
Conclusion: SD-4 can improve articular cartilage morphology and inflammation by regulating miRNA-140 and COL2 gene expressions, and thus ameliorating knee osteoarthritis of rabbits induced by Hulth’s method.

Abstract
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Author Biographies

Jingang, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia Autonomous Region, People’s Republic of China

Qimunge, International School of Mongolian Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia

Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia Autonomous Region, People’s Republic of China

Yanhua Xu

Affiliated Hospital of Inner Mongolia University for the Nationalities, Tongliao, Inner Mongolia Autonomous Region, People’s Republic of China

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Published

2023-06-30

How to Cite

Jingang, Qimunge, Hugejiletu, Hasitana, Xu, Y., Jigden, B., & Sharavyn, B. (2023). Effects of Mongolian Medicine Sengdeng-4 in Rabbit Knee Osteoarthritis Model. Central Asian Journal of Medical Sciences, 9(2), 42–49. https://doi.org/10.24079/cajms.2023.06.006

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