Results of HDV RNA detection in male seminal fluid and female cervical swab samples using RT-PCR

Authors

  • Saruul Enkhjargal Department of Chemical and Biological Engineering, School of Engineering and Technology, National University of Mongolia, Ulaanbaatar, Mongolia
  • Oyungerel Lkhagva-Ochir Department Clinical Research laboratory, Liver Center, Ulaanbaatar, Mongolia
  • Anir Enkhbat Department Clinical Research laboratory, Liver Center, Ulaanbaatar, Mongolia
  • Naranjargal Dashdorj Department Clinical Research laboratory, Liver Center, Ulaanbaatar, Mongolia
  • Nyamtsengel `Vangan Department of Infectiuos Diseases, School of Medicine, Mongolian National University of Medical Sciences, Ulaanbaatar, Mongolia
  • Odgerel Oidovsambuu Department of Chemical and Biological Engineering, School of Engineering and Technology, National University of Mongolia, Ulaanbaatar, Mongolia https://orcid.org/0000-0001-6872-3385

DOI:

https://doi.org/10.5564/pmas.v63i04.3432

Keywords:

HBV, HDV, Sexual transmission, Seminal fluid

Abstract

Mongolia has an extremely high prevalence of hepatitis D virus (HDV), which leads to increased morbidity and mortality from hepatocellular carcinoma (HCC). HDV is the most severe form of chronic viral hepatitis (CVH). A recent study estimated that the prevalence of anti-HDV was 67.5% among hepatitis B surface antigen-positive cases in Mongolia. HDV infection is particularly prevalent in sexually active young people. The virus may be transmitted through sexual intercourse, and it is crucial to confirm this by detecting HDV RNA in semen and cervical swabs of patients with chronic hepatitis delta. This pilot study was conducted with 16 participants, including eight males and eight females. Viral RNA was isolated from the blood, male seminal fluid, and female cervical swab samples of all participants in the study. HDV RNA was quantified using RT-PCR in samples from blood, seminal fluid, and cervical swabs. This study found that the detection rate of HDV RNA differed between male and female samples, with 62.5% of seminal fluid samples in the male cohort being positive compared with 87.5% in the female cohort. The data shows a statistically significant difference between genders for the presence of HDVRNA in seminal fluid and cervical swabs (OR 0.238, 95% CI= 0.02-3.01). A significant correlation was found between blood HDV RNA quantity and HDV RNA presence status in seminal fluid and cervical swab samples, indicating a potential risk of sexual HDV transmission. The R2 values for semen and cervical swab samples were 0.1095 and 0.9755, respectively.

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Published

2023-12-29

How to Cite

Enkhjargal, S., Lkhagva-Ochir, O., Enkhbat, A., Dashdorj, N., `Vangan, N., & Oidovsambuu, O. (2023). Results of HDV RNA detection in male seminal fluid and female cervical swab samples using RT-PCR. Proceedings of the Mongolian Academy of Sciences, 63(04), 27–36. https://doi.org/10.5564/pmas.v63i04.3432

Issue

Vol. 63 No 04 (248) 2023

Section

Articles

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Copyright (c) 2024 Saruul Enkhjargal, Oyungerel Lkhagva-Ochir, Anir Enkhbat, Naranjargal Dashdorj, Nyamtsengel `Vangan, Odgerel Oidovsambuu

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