Determination of triterpenoid saponin and polysaccharide content from in vitro cultures of Astragalus mongholicus Bunge
DOI:
https://doi.org/10.5564/pmas.v63i01.2804Keywords:
Astragalus mongholicus, in vitro cultures, secondary metabolite, total triterpenoid saponin, polysaccharideAbstract
In this study, the efficient micropropagation protocol of Astragalus mongholicus Bunge was established and also triterpenoid saponin and polysaccharide content in ethanol, methanol and aqueous extracts of different samples were determined by using spectrophotometric methods to investigate whether the content of biologically active compounds depends on the stage of development of the plant during in vitro culture. The content of total saponins and polysaccharides in different cultures of A. mongholicus grown in vitro was higher (990 and 505 μg/ml) in ethanol extracted 14-day-old young shoot samples than in 28-day-old propagated shoot samples and rooted shoots.
Downloads
220
References
Basalma, D., Uranbey, S., Gürlek, D. and Özcan, S. (2008). TDZ-induced Plant Regeneration in Astragalus cicer L. Afr. J. Biotechnol., 7(8): pp. 955-959.
Edreva. A., Velikova. V., Tsonev. T. (2008). Stress-protective role of secondary metabolites: diversity of functions and mechanisms. Gen Appl Plant Physiol. 34(1–2):67–78.
Edson JL, Wenny DL, Leege-Brusven A, Everett RL & Henderson DM (1994). Conserving threatened rare plants: some nursery strategies. National Proceedings, Forest and Conservation Nursery Associations. Fort Collins, CO: USDA For. Serv., Rocky Mtn. Forest and Range Exp. Sta., Gen. Tech. Rep. RMGTR-257:150-157.
Erisen, S., Yorgancilar, M., Atalay, E. and Babaoglu, M. (2009). Prolific Shoot Regeneration of Astragalus cariensis Boiss. Plant. Cell. Tiss. Org., 100(2):229–233. https://doi.org/10.1007/s11240-009-9638-3
Erisen, S., Yorgancilar, M., Atalay, E., Babaoglu, M. and Duran, A. (2010). Callus Induction and Plant Regeneration of the Endemic Astragalus nezaketae in Turkey. Electron. J. Biotechnol., 13(6):13-14. https://doi.org/10.2225/vol13-issue6-fulltext-3
Esmaeili, Gh., Azizi M., Aroei H., and Samiei L. (2016). Micropropagation of Astragalus adscendens: A Source of Gaz-angabin Manna in Iran (Persian Manna). J. Agr. Sci. Tech.18:741-750.
Freeman. BC., Beattie GA. (2008). An overview of plant defenses against pathogens and herbivores. Plant Health Instr. https://doi.org/10.1094/PHI-I-2008-0226-01
Grubov. V.I. (2008). Identification of Mongolian tuberous plants: Astragalus L. – Hunchir. Steel print, Ulaanbaatar, pp. 188-197.
Hartmann T. (2004). Plant-derived secondary metabolites as defensive chemicals in herbivorous insects: a case study in chemical ecology. Planta. https://doi.org/10.1007/s00425-004-1249-y
Hasançebi, S., Turgut Kara, N., Çakir, O. and Ari, S. (2011). Micropropagation and Root Culture of Turkish Endemic Astragalus chrysochlorus (Leguminosae). Turk. J. Bot., 35:203-210. https://doi.org/10.3906/bot-1007-48
Hou, S. W. and Jia, J. F. (2004). High Frequency Plant Regeneration from Astragalus melilotoides Hypocotyl and Stem Explants via Somatic Embryogenesis and Organogenesis. Plant. Cell. Tiss. Org., 79(1): 95–100. https://doi.org/10.1023/B:TICU.0000049445.69802.50
Jamiyandorj Kh., Ligaa U., Otgonbileg Kh., Saaral N.. (2011) Rare and very rare plant cultivation in Kherlen village. Ulaanbaatar. pp. 286-287.
Jiao J, Gai Q. Y, Fu Yu. J, Ma W, Yao L P, Feng Ch, Xia X.X (2015). Optimization of Astragalus membranaceus hairy roots induction and culture conditions for augmentation production of astragalosides. Plant Cell Tiss Organ Cult 120:1117–1130. https://doi.org/10.1007/s11240-014-0668-0
Kajimura. K., Takagi. Y., Miyano. K., Sawabe. Y., Mimura. M., Sakagami. Y., Yokoyama. H., Yoneda. K. (1997). Polysaccharide of Astragali radix enhances IgM antibody production in aged mice. Biological and Pharmaceutical Bulleten, 20:1178-1182. https://doi.org/10.1248/bpb.20.1178
Kim. J. H., Kim. M. H., Yang. G., Huh. Y., Kim. S. H., Yang. W. M. (2013). Effects of topical application of Astragalus membranaceus on allergic dermatitis. Immunopharmacology and. Immunotoxicology. 35:151-156. https://doi.org/10.3109/08923973.2012.733708
Kim. Y.S., Choi. Y. E., Sano. (2010). H. Plant vaccination: stimulation of defense system by caffeine production in planta. Plant Signal Behav. 5(5):489–93. https://doi.org/10.4161/psb.11087
Lee. D. Y., Noh. H. J., Choi. J., Lee. K. H., Lee. M. H, Lee. J. H., Hong. Y., Lee. S. H., Kim. S. Y., Kim. G. S. (2013). Anti-inflammatory cycloartane-type saponins of Astragalus membranaceus. Molecules, 18:3725-3732. https://doi.org/10.3390/molecules18043725
Li. Xi., Qu. L., Dong. Y., Han. L., Kiu. E., Fang. Sh., Zhang. Yi., Wang. T. (2014). A review of recent research progress on the Astragalus genus. Molecules, 19:18850-18880. https://doi.org/10.3390/molecules191118850
Liu, J., Chen, H. B., Guo, B. L., Zhao, Z. Z., Liang, Z. T., Yi, T. (2011). Study of the relationship between genetics and geography in determining the quality of Astragali Radix. Biol. Pharm. Bull. 34:1404–1412. https://doi.org/10.1248/bpb.34.1404
Liu. M., Wu. K., Mao. X., Wu. Y., Ouyang. J. (2009). Astragalus polysaccharide improves insulin sensitivity in KKAy mice: Regulation of PKB/GLUT4 signaling in skeletal muscle. Journal of Ethnopharmacology, 127:32-37. https://doi.org/10.1016/j.jep.2009.09.055
Luo J. P. & Jia J. F. (1998) Callus induction and plant regeneration from hypocotyl explants of the forage legume Astragalus adsurgens. Plant Cell Rep. 17:567–570. https://doi.org/10.1007/s002990050443
Ma. X., Zhang. K., Li. H., Han S., Ma. Z., Tu. P. (2013). Extracts from Astragalus membranaceus limit myocardial cell death and improve cardiac function in a rat model of myocardial ischemia. Journal of Ethnopharmacology, 149:720-728. https://doi.org/10.1016/j.jep.2013.07.036
Murthy, H. N, Dandin, V. S, Paek, K. Y, (2014). Tools for biotechnological production of useful phytochemicals from adventitious root cultures. Phytochem. Rev. 15:1–17. https://doi.org/10.1007/s11101-014-9391-z
Odontuya G, Odnyam R, Nomin M, Nomuun Ts, Enkhbat L, Undrah N. (2020). Towards standardization of cultivated two species of astragalus. Bulletin of the Institute of Chemistry and Chemical Technology, (8), pp. 1–7. https://doi.org/10.5564/bicct.v0i8.1470
Pistelli. L. (2002). Secondary metabolites of genus Astragalus: Structure and biological activity, Studies Natural Products Chemistry (ed. Atta-ur-Rahman). Elsevier Science, 27:443-545. https://doi.org/10.1016/S1572-5995(02)80043-6
Qi. H., Wei. L., Han. Y., Zhang. Q., Lau. A. S., Rong. J. (2010). Proteomic characterization of the cellular response to chemopreventive triterpenoid astragaloside IV in human hepatocellular carcinoma cell line HEPG2. International Journal of Oncology, 36:725-735. https://doi.org/10.3892/ijo_00000548
Smetanska. I. (2008). Production of secondary metabolites using plant cell cultures. Food Biotechnology.111:187-228. https://doi.org/10.1007/10_2008_103
Song J. Z, Yiu H. H. W, Qiao C. F, Han Q. B, Xu H. X. (2008). Chemical comparison and classification of Radix Astragali by determination of isoflavonoids and astragalosides. J. Pharm. Biomed. Anal. 47:399–406. https://doi.org/10.1016/j.jpba.2007.12.036
Southorn. I. W. (1994). In phytochemical dictionary of the Leguminosae (eds. Bisby F.A., Buckingham J., Harborne J.B.), Chapman & Hall, London, VI: pp. 87-107. https://doi.org/10.1007/978-1-4899-3047-7
Tian. Q. E., Li. H. D., Yan. M., Cai. H. L., Tan. Q. Y., Zhang. W. Y. (2012). Astragalus polysaccharides can regulate cytokine and P-glycoprotein expression in H22 tumor-bearing mice. World Journal of Gastroenterology. 18:7079-7086. https://doi.org/10.3748/wjg.v18.i47.7079
Tomoda. M., Shimizu. N., Ohara. N., Gonda. R., Ishi. S., Otsuki. H. (1992). A reticuloendothelial system-activating glycan from the roots of Astragalus membranaceus. Phytochemistry, 31:63-66. https://doi.org/10.1016/0031-9422(91)83006-7
Turgut Kara, N. and Ari, S. (2006). Micropropagation of Astragalus maximus willd. Biotechnol. Biotechnol. Equip., 20 (1):20-22.
Urgamal. M., Ouyntsetseg. B., Nyambayar. D., Dulamsuren. Ch (2014). Conspectus of the vascular plants of Mongolia. Ulaanbaatar, pp. 326.
Wang. D., Zhuamg. Y., Tian. Y., Thomas. G. N., Ying. M., Tomlinson. B. (2012). Study of the effects of total flavonoids of Astragalus on atherosclerosis formation and potential mechanisms. Oxidative Medicine and Cellular Longevity, https://doi.org/10.1155/2012/282383
Yorgancilar, M. and Erisen, S. (2011). The Effect of Thidiazuron (TDZ) on Shoot Regeneration of Astragalus schizopterus. J. Ani. Plant. Sci., 21(3): 519-524.
Yu. J., Zhang. Y., Sun. S., Shem. J., Qiu. J., Yin. X., Yin. H., Jiang. S. (2006). Inhibitory effects of astragaloside IV on diabetic peripheral neuropathy in rats. Canadian Journal of Physiology and Pharmacology. 84:579-587. https://doi.org/10.1139/y06-015
Zavattieri. M. A., Frederico. A. M., Lima. M., Sabino. R., Arnholdt-Schmitt B. (2010). Induction of somatic embryogenesis as an example of stress-related plant reactions. Electroni J Biotechnol. 13(1):12–3. https://doi.org/10.2225/vol13-issue1-fulltext-4
Zhang. D., Zhuang. Y., Pan. J., Wang. H., Li. H., Yu. Y., Wang. D. (2012). Investigaton of effects and mechanisms of total flavonoids of Astragalus and calycosin on human erythroleukemia cells. Oxidative Medicine and Cellular Longevity, 209843. https://doi.org/10.1155/2012/209843
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Tsolmon Munkhbayar, Batzaya Gachmaa, Azzaya Jukov, Oyunbileg Yungeree
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright on any research article in the Proceedings of the Mongolian Academy of Sciences is retained by the author(s).
The authors grant the Proceedings of the Mongolian Academy of Sciences a license to publish the article and identify itself as the original publisher.
Articles in the Proceedings of the Mongolian Academy of Sciences are Open Access articles published under a Creative Commons Attribution 4.0 International License CC BY.
This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
