Chemical constituents and antioxidant activity of Ranunculus japonicus Thunb.
DOI:
https://doi.org/10.5564/bicct.v13i13.5220Keywords:
Ranunculus japonicus Thunb, flavonoid, antioxidant activity, free radicals, DPPH, ABTSAbstract
We investigated the antioxidant activity and bioactive constituents of the aerial parts of Ranunculus japonicus Thunb., traditionally used in Mongolian medicine for wound healing and anti-inflammatory purposes. The dried plant material was extracted with 80% ethanol and the resulting crude extract was fractionated into dichloromethane, ethyl acetate, and n-butanol fractions. The antioxidant activity of these fractions was evaluated using DPPH and ABTS⁺ radical scavenging assays. Among them, the ethyl acetate fraction (DPPH I50 50.4±0.8 μg/mL; ABTS⁺ IC50 67.18±0.29 μg/mL) and the n-butanol fraction (DPPH IC50 83.9±3.4 μg/ mL; ABTS⁺ IC50 62.12±0.16 μg/mL) exhibited strong antioxidant activity whereas the ethanol extract and dichloromethane fraction showed low activity. The result of the activity guided isolation, two pure compounds were isolated from the active n-butanol fraction using chromatographic techniques. Their structures were elucidated by mass spectrometry and 1D/2D NMR spectroscopy and they were identified as quercetin-3-O-β-D-glucopyranoside (1) and apigenin-6-C-glucopyranoside (2). Both compounds demonstrated potent antioxidant activity, with compound 1 showing higher efficacy than the positive control, scavenging 61.97–81.02% of DPPH and ABTS⁺ radicals at 25 μg/mL concentration (DPPH IC50 14.61±0.14 μg/mL; ABTS⁺ IC50 18.79±0.19 μg/mL). These findings indicate that R. japonicus is a promising natural source of potent antioxidants and provide scientific support for its traditional medicinal use.
Наран холтсон цэцэг (Ranunculus japonicus Thunb.) ургамлын антиоксидант идэвх болон зарим химийн бүрэлдэхүүний судалгаа
Хураангуй: Бид Монголын уламжлалт анагаах ухаанд шарх эдгээх, үрэвсэл дарах зорилгоор хэрэглэдэг наран холтсон цэцэг (Ranunculus japonicus Thunb.) ургамлын газрын дээд хэсгийн антиоксидант идэвх болон идэвхт нэгдлийг илрүүлэх, цэврээр ялган бүтцийг тогтоох судалгааг хийж гүйцэтгэлээ. Наран холтсон цэцэг ургамлын хатаасан дээжийг 80% этилийн спиртээр хандалж этанолын ханд түүнээс туйлгүй дихлорметан, туйлт этилацетат болон н-бутанолын бүлэг хандыг гарган авч антиоксидант идэвхийг DPPH болон ABTS⁺ радикал зайлуулах аргуудаар судлав. Судалгааны дүнд туйлт этилацетат (DPPH радикал IC50 50.4±0.8 мкг/мл, ABTS+ радикал IC50 67.18±0.29 мкг/мл) болон н-бутанолын (DPPH радикал IC50 83.9±3.4 мкг/мл, ABTS+ радикал IC50 62.12±0.16 мкг/мл) бүлэг ханд хүчтэй антиоксидант идэвх үзүүлсэн бол этанолын ханд, дихлорметаны бүлэг ханд идэвхгүй байв. Иймд хүчтэй идэвх үзүүлсэн н-бутанолын бүлэг ханднаас хроматогоафийн аргаар хоёр цэвэр нэгдлийг ялгасан ба бүтцийг масс спектр ба 1D/2D ЦСР спектроскопын аргаар судлан кверцетин-3-O-β-D -глюкопиранозид (1) болон апигенин-6-C-глюкопиранозид (2) нэгдлүүд болохыг тогтоов. Цэврээр ялгасан нэгдлүүд хүчтэй антиоксидант идэвх үзүүлсэн ба ялангуяа нэгдэл 1 эерэг стандарттай харьцуулахад илүү идэвхтэй буюу 25 мкг/мл тундаа DPPH болон ABTS+ радикалуудыг 61.97-81.02%-р зайлуулж байв (IC50 14.61±0.14 мкг/мл, ABTS+ радикал 18.79±0.19 мкг/ мл). Эдгээр үр дүн нь наран холтсон цэцэг ургамал нь байгалийн гаралтай өндөр идэвхтэй антиоксидантын чухал эх үүсвэр байж болохыг харуулж, уламжлалт хэрэглээний шинжлэх ухааны үндэслэлийг бататгав.
Түлхүүр үг: Наран холтсон цэцэг, флавоноид, антиоксидант, чөлөөт радикал, DPPH, ABTS
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