Biological activity and toxicity of extracts from Artemisia sieversiana Willd
DOI:
https://doi.org/10.5564/bicct.v10i10.1803Keywords:
Brine shrimp, Artemisia, S.aureus, plant extracts, wormwoodAbstract
In our study, we determined the biochemical and biological activity and toxicity of Artemisia sieversiana Willd., which grows in Mongolia. The amount of moisture, acidity, protein, oil, total and crude carbohydrates, vitamins and secondary metabolites in the dry weight of the plant was determined. A group of extracts was taken out and the chemical compounds of each group were identified by thin layer chromatography, and antioxidant activity was determined, IC50 value was calculated. IC50 values were calculated for hexane extract >200 μg/ml, chloroform extract 62.2 μg/ml, ethyl-acetate extract 54.8 μg/ml, n-butanol extract 55.9 μg/ml, aqueous extract 84.34 μg/ml. Ethylacetate, chloroform and hexane extracts showed growth inhibition zones for S.aureus ATCC2592 and B. subtilis ATCC6633. Each group of extracts was prepared at four different concentrations and the toxicity to Artemia salina crab larvae was determined and the LC50 value was calculated by Clarkson's toxicity index.
Cеверсийн шарилж (Аrtemisia sieversiana Willd.)-ийн хандны биологийн идэвх, хорон чанарын судалгаа
Хураангуй: Бид судалгаандаа Монгол оронд ургадаг Северсийн шарилж (Artemisia sieversiana Willd.)-ийн биохими болон биологийн идэвх, хорон чанарыг тодорхойлов. Үүнд ургамлын хуурай жинд чийг, хүчиллэг, уураг, тос, нийт болон ангижирсан нүүрс ус, витамин, хоёрдогч метаболитуудийн хэмжээг тодорхойлов. Бүлэг хандуудыг гарган авч нимгэн үеийн хроматографийн аргаар бүлэг ханд тус бүрийн химийн нэгдлүүдийг таньж, антиоксидант идэвхийг тодорхойлон IC50 утгыг тооцоход гексан ханд >200 мкг/мл, хлороформын ханд 62.8 мкг/мл, этил-ацетатын ханд 54.8 мкг/мл, н-бутанолын ханд 55.9 мкг/мл, усан ханд 84.34 мкг/мл. Этил-ацетат, хлороформ, гексан ханд S.aureus ATCC2592, B.subtilis ATCC6633 бактерийн өсөлтийг дарангуйлах ариун бүс үүсгэв. Бүлэг ханд тус бүрийг дөрвөн өөр концентрацтайгаар бэлтгэж хорон чанарыг Artemia salina хавчийн авгалдайд тодорхойлон, LC50 утгыг тооцож Кларксоны хорон чанарын индексээр үнэлэхэд этилацетатын ханд 414.43 мкг/мл буюу дунд зэргийн хортой бусад ханд хоргүй байв.
Түлхүүр үг: Brine shrimp, царван, S.aureus, ургамлын хоёрдогч метаболит, шарилж
Downloads
167
References
Ш.Даариймаа (1987). Шарилжийн төрлийн ургамлын Монгол орны ургамлын нөмрөгт гүйцэтгэх үүрэг. ШУ, Ботаникийн хүрээлэнгийн бүтээл.3:31-35.
У.Лигаа, Б.Даваасүрэн, Н.Нинжил (2005). Монгол орны эмийн ургамлыг өрнө дорнын анагаах ухаанд хэрэглэхүй. Улаанбаатар. 11, 274.
S.J.Liu, Z.X.Liao, Z.S.Tang, C.L.Cui, H.Bo Liu, Y.N.Liang, Y.Zhang, H.X.Shi, Y.R.Liu (2017). Phytochemicals and biological activities of Artemisia sieversiana. Phytochemistry Reviews. 16(3):441-460. https://doi.org/10.1007/s11101-016-9475-z.
J.Kumar, G.P.Mishra, P.K.Naik, A.A.Murkute, R.B.Srivastava (2011). Genomic DNA isolation from Artemisia species grown in cold desert high altitude of India. African Journal of Biotechnology. 10:7303–7307. https://doi.org/10.5897/AJB10.2031
E.M.Suleimenov, F.M.Smagulova, R.B.Seidakhmetova, R.M.Aksartov, V.A.Raldugin, S.M.Adekenov (2007) Epiashantin from Artemisia sieversiana. Chemistry of Natural Compdounds. 43:232-233. https://doi.org/10.1007/s10600-007-0090-5
М.ИГоряев, В.СБазалицкая, П.ППоляков (1962). Химический состав Полыней, Издательство Акатемий Наук Казахской ССР, 48-49.
Ц.Энхтуяа, Х.Дэлгэр (2011). Баруун бүсийн малын маханд хлорамфеникол, авермектиний үлдэгдэл илрүүлэх тандалдын дүн, оношлох дэвшилтэд арга ОҮБХ-нийтлэл. Улаанбаатар. 33.
Б.Эрдэнэчулуун, Н.Ганчимэг, Э.Жаргалхүү (2013). Чих хамар хоолой судлал. Эрүүл мэндийн шинжлэх ухааны их сургууль. Улаанбаатар. 95-101.
В.И.Грубов (2008). Монголын гуурст ургамлыг таних бичиг. Ган принт. Улаанбаатар. 297.
Ж.Сүхдолгор (2008). Ургамлын хими, биохими дадлага ажлын дэвтэр, Ган принт хэвлэлийн компани. УБ. 37-38.
Г.Даваахүү, Б.Батжаргал (2018). Биохимийн хичээлийн лабораторийн дэвтэр-II. МУИС пресс хэвлэлийн газар. УБ. 42-43, 45-53.
S.Hadi, J.B.Bremner (2001). Initial studies on alkaloids from Lombok medicinal plants. Molecules. 1. 6:117-129. https://doi.org/10.3390%2F60100117
R.Madaan, G.Bansal, S.Kumar, A.Sharma (2011). Estimation of total phenols and flavonoids in extracts of Actaea spicata roots and antioxidant activity studies. Indian Journal of Pharmaceutical Sciences. 73(6):666-9. https://doi.org/10.4103/0250-474x.100242
M.D.Bois, K.A.Gilles, J.K.Hamilton, P.A.Rebers, F.Smith (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry. 28(3):350-356. https://doi.org/10.1021/ac60111a017
T.Masuko, A.Minami, N.Iwasaki, T.Majima, S.I.Nishimura, Y.C.Lee (2005). Carbohydrate analysis by a phenol–sulfuric acid method in microplate format. Analytical Biochemistry. 339(1):69-72. https://doi.org/10.1016/j.ab.2004.12.001
A.W.Bauer, W.M.M.Kirby, J.C.Sherris, M.Turck (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology. 45:493–496.
L.L.Mensor, S.F.Menezes, G.G.Leitão, S.A.Reis, T.C.dos Santos, C.G.Coube, S.Leitão (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy Research. 15(2):127-130. https://doi.org/10.1002/ptr.687
M.S.Blois (1958). Antioxidant determinations by the use of a stable free radical. Nature. 181:1199-1200. http://dx.doi.org/10.1038/1811199a0
C.Clarkson, V.J.Maharaj, N.R.Crouch, O.M.Grace, P.Pillay, M.G.Matsabisa, N.Bhagwandin, P.J.Smith, P.I. Folb (2004). In vitro antiplasmodial activity of medicinal plants native to or naturalized in South Africa. Journal of Ethnopharmacology. 92:177–191. https://doi.org/10.1016/j.jep.2004.02.011
Ж.Доржбат, М.Бямбадолгор, Б.Амаржаргал, Б.Насантогтох, Ж.Сүхдолгор, Д.Лхагвасүрэн, Т.Сувдмаа (2021). Нангиад ороонго (Cuscuta chinensis Lam)-ын биологийн идэвх, хорон чанарын судалгаа. Bulletin of the Institute of Chemistry and Chemical Technology. 9:40-46. https://doi.org/10.5564/bicct.v4i9.1817
Z.L.Liu, Q.R.Liu, S.S.Chu, G.H.Jiang (2010). Insecticidal activity and chemical composition of the essential oils of Artemisia lavandulaefolia and Artemisia sieversiana from China. Chemistry and Biodiversity. 7:2040-2045. https://doi.org/10.1002/cbdv.200900410
H.Jork, W.Funk, W.Fischer, H.Wimer (1994). Thin layer chromatography. Reagents and detection methods. Weinheim: VCH Verlagsgesellschaft mbH. 124-undefined. https://doi.org/10.1016/S0003-2670%2800%2983962-8
E.De.Rijke, P.Out, W.M.A.Niessen, F.Ariese, C.Gooijer, U.A.T.Brinkman (2006). Analytical separation and detection methods for flavonoids. Journal Chromatography A. 111(2):31–36. https://doi.org/10.1016/j.chroma.2006.01.019
J.Cui, M.Nuermaimaiti, G.Zou, X.Xin (2020). Chemical composition of Artemisia sieversiana. Chemistry of Natural Compdounds. 56:1120-1121.
M.Ayub, S.Hussain, A.Rasool, I.Hussain, F.Khan, A.Sultan (2019). Insecticidal activity of Artemisia sieversiana Ehrh. plant extract against white grub (Scarabaeidae: Coleoptera). Journal of Entomology and Zoology Studies 7:304-308.
R.X.Tan, W.F.Zheng, H.Q.Tang (1998). Biologically active substances from the genus Artemisia. Planta Medica. 64:295–302. https://doi.org/10.1055/s-2006-957438
J.Tang, J.J.Zhao, Z.H.Li (2015). Ethanol extract of Artemisia sieversiana exhibits anticancer effects and induces apoptosis through a mitochondrial. Bangladesh Journal of Pharmacol. 10:518-523. https://doi.org/10.3329/bjp.v10i3.23196
M.Nakai, M.Harada, K.Nakahara, K.Akimoto, H.Shibata, W.Miki, Y.Kiso (2003). Novel antioxidative metabolites in rat liver with ingested sesamin. Journal of Agricultural Food Chemistry. 51:1666-1670. https://doi.org/10.1021/jf0258961
J.Kang, Z.Li, T.Wu, G.S.Jensen, A.G.Schauss, X.Wu (2010). Anti-oxidant capacities of flavonoid compounds isolated from acai pulp (Euterpe oleracea Mart.). Food Chem. 122:610-617.
S.J.Liu, Z.X.Liao, C.Liu, L.J.Ji, H.F.Sun (2014). Two new sesquiterpenes from Artemisia sieversiana. Fitoterapia. 97:43-49. https://doi.org/10.1016/j.fitote.2021.104996
D.R.Ruebhart, I.E.Cock, G.R.Shaw (2008). Brine shrimp bioassay: importance of correct taxonomic identification of Artemia (Anostraca) species. Environmental Toxicology. 23(4):555–560. https://doi.org/10.1002/tox.20358
C.R.Rafael, M.M.Alejandro M, O.R.Hortencia, M.Gopal, G.T.Danitzia A, M.S.Pablo (2003). Mixture of parthenogenetic and zygogenetic brine shrimp Artemia (Branchiopoda: Anostraca) in commercial cyst lots from Great Salt Lake, UT, USA. Journal of Experimental Marine Biology and Ecology. 296 (2):243–251. https://doi.org/10.1016/S0022-0981(03)00339-3
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Amarbayasgalan Altantuul, Janchivmaa Dorjbat, Baatarkhuu Amarjargal, Bazarvaani Nasantogtokh, Purevsuren Sarantsetseg, Tuvaanjav Suvdmaa
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright on any research article in the Bulletin of the Institute of Chemistry and Chemical Technology, MAS is retained by the author(s).
The authors grant the Bulletin of the Institute of Chemistry and Chemical Technology, MAS a license to publish the article and identify itself as the original publisher.
Articles in the Bulletin of the Institute of Chemistry and Chemical Technology, MAS are Open Access articles published under a Creative Commons Attribution 4.0 International License CC BY.
This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
