Bacteriolytic activity of phages isolated from wastewater
DOI:
https://doi.org/10.5564/pib.v39i2.3327Keywords:
Bacteriophage, bacteriolytic activity, bacteria, E. coli, P. aeruginosa, wastewaterAbstract
Bacteriophages, which are viruses that infect bacteria, have a significant impact on the dynamics of bacterial communities, the evolution of bacterial genomes, and the biogeochemistry of ecosystems. The specific effects of these factors differ based on whether the phages establish lytic, chronic, or lysogenic infections. In this study, bacteriophages were isolated from wastewater samples obtained from the Central Wastewater Treatment Plant of Ulaanbaatar. The test strains of Escherichia coli NIM05 and Pseudomonas aeruginosa NIM09 from the collection of the Bacteriological Laboratory, the National Centre for Infectious Diseases were used to determine bacteriolytic activity of bacteriophages. Bacteriophages AT1 and AT2, demonstrating bacteriolytic activity, were isolated. Specifically bacteriophage AT1 was able to lyse 70.2% of cells from the total population of E. coli NIM05, while bacteriophage AT2 was able to lyse 78.5% of P. aeruginosa NIM09 within a 96-hour cultivation period. Bacteriophage titers were determined, resulting in 1.12 x 1010 PFU/m for the AT1 phage, and 0.98 x 1010 PFU/mL for the AT2 phage.
Бохир уснаас ялган авсан фагийн бактериолитик идэвх
Хураангуй. Бактерид халдварладаг вирус нь бактерийн өсөлтийн динамик, бактерийн геномын хувьсал, экосистемийн биогеохимийн процесст нөлөөлдөг. Эдгээр нөлөөллүүд нь тухайн фагууд литик, архагшсан эсвэл лизоген халдвар үүсгэж байгаа эсэхээс хамаарч өөр өөр байдаг. Энэхүү судалгаанд ХӨСҮТ –ийн Нэгдсэн лабораторийн Нян судлалын лабораторид хадгалагдаж байгаа Escherichia coli NIM05 болон Pseudomonas aeruginosa NIM09 –ийн омгуудыг тест өсгөврөөр ашиглав. Улаанбаатар хотын Төв цэвэрлэх байгууламжийн бохир уснаас эдгээр өсгөврийг лизист оруулах чадвартай АТ1 ба АТ2 бактериофагуудийг ялгаж авав. АТ1 бактериофаг E. coli –ийн нийт эсийн 70.2% –ийг, AT2 фаг P. аeruginosa –ийн нийт эсийн 78.5.5% –ийг 96 цагийн хугацаанд лизист оруулах чадвартай болохыг тогтоов. Ялгаж авсан AT1 фагийн титр 1.12x1010 PFU/mL, AT2 фагийн титр 0.98x1010 PFU/mL тус тус байна.
Түлхүүр үгс: Бактериофаг, бактериолитик идэвх, бактери, E. coli, P. aeruginosa, бохир ус
Downloads
60
References
Ackermann, H.W., (2009). Phage classification and characterisation. Methods. Mol. Biol, 501, 127-140. https://doi.org/10.1007/978-1-60327-164-6_13
Ackermann, H.W. and DuBow, M.S. (1987). Viruses of Prokaryotes: General Properties of Bacteriophages; CRC Press Inc.: Boca Raton, FL, USA, Volume 1, pp. 49-85.
Ashelford, K.E., Norris, S.J., Fry, J.C., Bailey, M.J. and Day, M.J. (2000). Seasonal population dynamics and interactions of competing bacteriophages and their host in the rhizosphere. Applied and Environmental Microbiology, vol. 66, no. 10, pp. 4193-4199. https://doi.org/10.1128/AEM.66.10.4193-4199.2000
Beheshti Maal, K., Soleimani Delfan, A. and Salmanizadeh, S. (2015). Isolation and Identification of Two Novel Escherichia coli bacteriophages and their application in wastewater treatment and coliform's Phage Therapy. Jundishapur Journal of Microbiology, vol. 8, no. 3, pp. e14945. https://doi.org/10.5812/jjm.14945
Chen, Y., Sun, E., Song, J. et al., (2018). Complete genome sequence of a novel T7-Like bacteriophage from a Pasteurella multocida capsular type A isolate. Curr. Microbiol. 75:574-579. https://doi.org/10.1007/s00284-017-1419-3
DeBritto, S., Gajbar, T.D., Satapute, P. et al., (2020). Isolation and characterization of nutrient-dependent pyocyanin from Pseudomonas aeruginosa and its dye and agrochemical properties. Sci. Rep. 10, 1542 https://doi.org/10.1038/s41598-020-58335-6.
Fauquet, C.M., (2008). Taxonomy, Classification and Nomenclature of Viruses. Encyclopedia of Virology, 9-23. https://doi:10.1016/B978-012374410-4.00509-4.
Geneva: World Health Organization., (2020). Global antimicrobial resistance surveillance system (glass) report: early implementation 2020.
Howard-Varona, C., Hargreaves, K., Abedon, S. et al., (2017). Lysogeny in nature: mechanisms, impact and ecology of temperate phages. ISME J 11, 1511-1520 https://doi.org/10.1038/ismej. 2017.16.
Hyman, P. and Abedon, S.T. (2010). Bacteriophage host range and bacterial resistance. Advances in Applied Microbiology, vol. 70, pp. 217-248. https://doi.org/10.1016/S0065-2164(10)70007-1
Lau, G.W. et al. (2004). Pseudomonas aeruginosa pyocyanin is critical for lung infection in mice. Infect. Immun. 72, 4275-4278. https://doi.org/10.1128/IAI.72.7.4275-4278.2004
Mattila, S., Ruotsalainen, P., Jalasvuori, M., (2015). On-demand isolation of bacteriophages against drug-resistant bacteria for personalized phage therapy. Front. Microbiol., 6, 1271. https://doi.org/10.3389/fmicb.2015.01271.
Monk, A., Rees, C., Barrow, P., Hagens, S. and Harper, D. (2010). Bacteriophage applications: where are we now? Letters in Applied Microbiology, vol. 51, no. 4, pp. 363-369. https://doi.org/10.1111/j.1472-765X.2010.02916.x
Rasool, M.H., (2016). Isolation, Characterization, and Antibacterial Activity of Bacteriophages Against Methicillin-Resistant Staphylococcus aureus in Pakistan. Jundishapur Journal of Microbiology, 9(10). https://doi.org/10.5812/jjm.36135.
Sharp, R. (2001). Bacteriophages: biology and history. Journal of Chemical Technology and Biotechnology (Oxford, Oxfordshire), vol. 76, no. 7, pp. 667-672. https://doi.org/10.1002/jctb.434
Safia Samir, A.E., (May 2022). Isolation and characterization of lytic bacteriophages from sewage at an egyptian tertiary care hospital against methicillin-resistant Staphylococcus aureus clinical isolates. Saudi Journal of Biological Sciences Volume 29. Issue 5. Pages 3097-3106. https://doi.org/10.1016/j.sjbs.2022.03.019.
Saleem, H., Mazhar S, Syed Q, Javed MQ, Adnan A., (2021). Bio-characterization of food grade pyocyanin bio-pigment extracted from chromogenic Pseudomonas species found in Pakistani native flora. Arab J. Chem. 14(3):103005. https://doi.org/10.1016/j.arabjc.2021.103005.
Tumenjargal, D., Khulan, J., Anujin, G., Khaliunaa, T., Ochirkhuyag, B., (2023). A study on the detection of bacteriophages able to lyse Staphylococcus aureus and Enterococci. Ecology and Geochemical Activity of Microorganisms in Extreme Environments, 3rd all-Russian conference with international participation, Ulan-Ude - Baikalsk, July 3-7, 2023, Book of abstracts, Novosibirsk, 2023: pp.124.
Wardani, A. K., Nurbayu, I. R., Qodriyah, N.L., (2020). Isolation of lytic bacteriophages and their potential to control Cronobacter spp. opportunistic food-borne pathogens. IOP Conference Series: Earth and Environmental Science, 475, 012086-. https://doi.org/10.1088/1755-1315/475/1/012086.
World Health Organization. (accessed 15 January 2015). The top 10 causes of death. http://www.who.int/mediacentre/factsheets/fs310/en/index1.html
Downloads
Published
How to Cite
License
Copyright (c) 2023 Gantulga Anujin, Janchiv Khulan, Baldorj Ochirkhuyag, Davaasuren Tumenjargal
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright on any research article in the Proceedings of the Institute of Biology is retained by the author(s).
The authors grant the Proceedings of the Institute of Biology license to publish the article and identify itself as the original publisher.
Articles in the Proceedings of the Institute of Biology are Open Access articles published under a Creative Commons Attribution-NonCommercial 4.0 International License - CC BY NC.
This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
