Study of endophytic bacteria isolated from endangered and rare medicinal plants in Mongolia
DOI:
https://doi.org/10.5564/pib.v39i2.3326Keywords:
endophytic bacteria, identification, rare and endangered medicinal plant, indole acetic acid, antagonistic activityAbstract
A total of 41 strains of endophytic bacteria were isolated from 6 species of rare and endangered medicinal plants in Mongolia. Among them, 10 strains produced indole acetic acid (IAA), 8 and 3 strains were positive for phosphate and zinc solubilization, respectively. Out of the strains capable of producing of IAA, and solubilizing phosphate and zinc, the strains P23-R2 and P26-H1, isolated respectively from the root of Glycyrrhiza uralensis and aboveground parts of Cynomorium songaricum, demonstrated the ability to produce IAA and solubilize phosphate. In addition, the strains P31-F1, and P31-L1, isolated from the flowers and leaves of Lancea tibetica, were positive for solubilizing both phosphate and zinc. Besides, the antagonistic activity of the total isolates was tested, and one strain exhibited antagonistic activity against E. coli, one against S. aureus, two against B. subtilis, three strains showed activity against C. albicans and one against A. niger. The strain P26-H5, isolated from the above part of Cynomorium songaricum, exhibited antagonistic activity against 3 test organisms; E. coli (18.5 mm), C. albicans (10.5 mm), A. niger (20.2 mm). A molecular marker was used for the identification of taxonomic relationship of 23 strains capable of producing IAA, phosphate and zinc solubilization, as well as exhibiting antagonistic activity. The result revealed that the strains belonged to 9 distinct genera: Acinetobacter, Alcaligenes, Bacillus, Klebsiella, Paenibacillus, Peribacillus, Pseudomonas, Serratia, Terribacillus. The Klebsiella sp. P26-H1 resulted the highest production of IAA (28.14 – 68.02 µg/ml), suggesting that it could be used in the future to promote the growth of agricultural plants.
Монгол орны нэн ховор, ховор эмийн ургамлаас ялгасан эндофит бактерийн судалгаа
Хураангуй. Монгол орны нэн ховор, ховор 6 зүйл эмийн ургамлаас эндофит бактерийн 41 өсгөвөр ялган авсан. Эдгээр өсгөврүүдээс 10 өсгөвөр индол цууны хүчил (ИЦХ) нийлэгжүүлж, 8 өсгөвөр фосфат, 3 өсгөвөр цайрыг уусгах чадвартай байв. ИЦХ нийлэгжүүлж, фосфат болон цайр уусгах чадвартай байсан өсгөврүүдээс Урал чихэр өвсний үндэснээс ялгасан P23-R2, Зүүн гарын гоёоны газрын дээд хэсгээс ялган авсан P26-H1 дугаартай өсгөврүүд нь ИЦХ нийлэгжүүлэхээс гадна фосфат уусгах чадвартай байна. Мөн Төвд ланцайны цэцэг болон навчнаас ялгасан P31-F1, P31-L1 өсгөврүүд нь фосфат болон цайрыг уусгах чадвартай байв. Цаашид нийт ялган авсан өсгөврүүдийн антагонист идэвхийг тогтооход E. coli-ийн эсрэг 1, S. aureus-ийн эсрэг 1, B. subtilis-ийн эсрэг 2, C. albicans-ийн эсрэг 3, A. niger-ийн эсрэг 1 өсгөвөр идэвхтэй байв. Тэдгээрээс Зүүнгарын гоёоны газрын дээд хэсгээс ялган авсан P26-H5 өсгөвөр нь E. coli (18.5 мм), C. albicans (10.5 мм ), A. niger (20.2 мм)-ийн эсрэг идэвхтэй байна. ИЦХ нийлэгжүүлэх, фосфат, цайр уусгах, чадвартайгаас гадна антагонист идэвхтэй 23 өсгөврийн ангилалзүйн хамаарлыг молекул маркер ашиглан тогтооход Acinetobacter, Alcaligenes, Bacillus, Klebsiella, Paenibacillus, Peribacillus, Pseudomonas, Serratia, болон Terribacillus зэрэг нийт 9 өөр өөр төрөлд хамаарагдаж байна. Klebsiella sp. P26-H1 өсгөвөр нь хамгийн их буюу 28.14 – 68.02 мкг/мл ИЦХ нийлэгжүүлж байгаа нь цаашид газар тариалангийн ургамлын өсөлтийг дэмжихэд ашиглах боломж буйг харуулж байна.
Түлхүүр үгс: эндофит бактери, тодорхойлох, нэн ховор, ховор эмийн ургамал, индол цууны хүчил,
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