Biocatalytic properties of horseradish root extract peroxidase (HRP)

Authors

  • D Purev Department of Biology, School of Science, NUM, Ulaanbaatar
  • J Bayarmaa Department of Biology, School of Science, NUM, Ulaanbaatar
  • S Tsevelmaa Department of Biology, School of Science, NUM, Ulaanbaatar
  • A Zolzaya Medical Bioengineering Department, Etugen Instutite, Ulaanbaatar

DOI:

https://doi.org/10.5564/mjc.v15i0.332

Keywords:

Horseradish peroxidase (HRP), enzyme activity, biocatalytic properties

Abstract

The objective of the present work was to examine biocatalytic properties of peroxidase in horseradish acclimatized in our country. We have found that horseradish root extract’s peroxidase (HRP) has Km 2.5 mM and Vmax 5.36 mM·s-1. Maximum activity (pHopt) was estimated at pH 6.0 and enzyme is more stable in alkali, than in acid. The optimum temperature (Topt) for HRP is 40oC and the enzyme is not stable to temperature influence. The horseradish  root’s extract retains enzymatic activity within 21 days.

DOI: http://doi.dx.org/10.5564/mjc.v15i0.332

Mongolian Journal of Chemistry 15 (41), 2014, p101-103

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Published

2014-12-12

How to Cite

Purev, D., Bayarmaa, J., Tsevelmaa, S., & Zolzaya, A. (2014). Biocatalytic properties of horseradish root extract peroxidase (HRP). Mongolian Journal of Chemistry, 15, 101–103. https://doi.org/10.5564/mjc.v15i0.332

Issue

Vol. 15 (2014)

Section

Articles

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