HETEROLOGOUS EXPRESSION OF ANTIMICROBIAL PEPTIDE LL-37 IN CHINESE CABBAGE WITH ENHANCED RESISTANCE TO PATHOGENS

Authors

  • Bayartbat Ishvaanjil Mongolian University of Life Science, Ulaanbaatar
  • Yu-Jin Jung Hankyong National University, Ansung, South Korea
  • Uyangaa Temuujin Mongolian University of Life Science, Ulaanbaatar
  • Soon-Youl Lee Hankyong National University, Ansung, South Korea
  • Kwon-Kyoo Kang Hankyong National University, Ansung, South Korea

DOI:

https://doi.org/10.5564/mjas.v13i2.531

Keywords:

Brassica rapa, Chinese cabbage, Agrobacterium tumefaciens, LL-37, resistance, pathogen

Abstract

The human antimicrobial peptide, LL-37 gene was overexpressed in Chinese cabbage ‘Osome’ (Brassica rapa) by Agrobacterium tumefaciens-mediated transformation. In order to increase the expression of the antimicrobial peptide, we used RolA intron sequence in front of the LL-37peptide gene. We confirmed the expression of LL-37 in cabbage by RT-PCR and Western Blot analysis. Four transgenic T1 plants were confirmed that LL-37 was expressed. Cabbages expressing the humanLL-37 gene were challenged by various plant pathogen. Transgenic cabbage plants overproducing human LL-37 are expected to possess a durable and wide-spectrum resistance against various pathogens.

Mongolian Journal of Agricultural Sciences Vol.13(2) 2014: 124-130

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Published

2015-06-22

How to Cite

Ishvaanjil, B., Jung, Y.-J., Temuujin, U., Lee, S.-Y., & Kang, K.-K. (2015). HETEROLOGOUS EXPRESSION OF ANTIMICROBIAL PEPTIDE LL-37 IN CHINESE CABBAGE WITH ENHANCED RESISTANCE TO PATHOGENS. Mongolian Journal of Agricultural Sciences, 13(2), 124–130. https://doi.org/10.5564/mjas.v13i2.531

Issue

Vol. 13 No. 2 (2014)

Section

Articles

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