Characterization and antimicrobial susceptibility of Escherichia coli, Salmonella enterica isolated from Uvurkhangai province in Mongolia
DOI:
https://doi.org/10.5564/mjas.v16i38.1545Keywords:
Enterobacteriaceae, AMR, colibacteriosis, salmonellosisAbstract
The incidence of infectious diseases caused by the Enterobacteriaceae family of Uvurkhangai province in Mongolia is not decreasing and is recurring despite the implementation of preventive measures in the province. Nowadays, when the resistance of microorganisms negatively affects the health of humans and animals, food safety, the environment, and the economy, the lack of effective antibiotics for treatment is the reason for the research work on this topic. Based on the outbreak and surveillance of infectious diseases for the past 5 years, the pathological materials were received in the veterinary laboratory, two strains of Escherichia coli and also two strains Salmonella enterica subsp. abortus equi were isolated, and identified by conventional bacteriological and molecular methods. The antimicrobial susceptibility of the pathogen was determined using the disc diffusion method. The strains of E. coli were resistant to several antimicrobials, including vancomycin and neomycin, intermediate to colistin. The Salmonella enterica subsp. abortus equi showed resistance to ampicillin, vancomycin, gentamicin, and neomycin. Due to the presence of antimicrobial resistance, the results suggest a risk to public health as these pathogens. However, the four isolated bacteria in the family of Enterobacteriaceae showed susceptibility to groups of tetracycline and streptomycin. As a result of this study, it was determined that treatment with tetracycline and streptomycin is effective against bacterial infections caused by the Enterobacteriaceae group.
Downloads
169
References
National Statistics Office, "Number of livestock," https://1212.mn/mn. Accessed: Feb. 22, 2024. [Online]. Available: https://1212.mn/mn
E. Grandolfo et al., "High mortality in foals associated with Salmonella enterica subsp. enterica Abortus equi infection in Italy," Journal of Veterinary Diagnostic Investigation, vol. 30, no. 3, pp. 483-485, May 2018. https://doi.org/10.1177/1040638717753965
C. P. Bustos et al., "Salmonella enterica serovar Abortusequi as an emergent pathogen causing equine abortion in Argentine," J Equine Vet Sci, vol. 39, pp. S56-S65, Apr. 2016. https://doi.org/10.1016/j.jevs.2016.02.127
R. L. dos Santos et al., "Molecular characterization and biofilm-formation analysis of Listeria monocytogenes, Salmonella spp., and Escherichia coli isolated from Brazilian swine slaughterhouses," PLoS One, vol. 17, no. 9 September, Sep. 2022. https://doi.org/10.1371/journal.pone.0274636
H. Wang et al., "Abortion in donkeys associated with Salmonella abortus equi infection," Equine Vet J, vol. 51, no. 6, pp. 756-759, Nov. 2019. https://doi.org/10.1111/evj.13100
WOAH, "Chapter 3.10.7 Salmonellosis," WOAH Terrestrial Manual 2022.
State Central Veterinary Laboratory, "Colibacteriosis." Accessed: Feb. 22, 2024. [Online]. Available: http://scvl.gov.mn/application/newsdetail?newsId=129
A. M. Lupindu, "Isolation and Characterization of Escherichia coli from Animals, Humans, and Environment," in Escherichia coli - Recent Advances on Physiology, Pathogenesis and Biotechnological Applications, InTech, 2017. https://doi.org/10.5772/67390
H. Kodaka, Y. Uesaka, and F. Kashitani, "Erratum: Nissui Glucose Fermentative Gram-Negative Rod Identification System EB-20 Gives a Unique Profile for Typical Non-Sorbitol-Fermenting Escherichia coli O157:H7 (Journal of Clinical Microbiology (2004) 42, 1 (354-358))," Journal of Clinical Microbiology, vol. 42, no. 4. p. 1857, Apr. 2004. https://doi.org/10.1128/JCM.42.4.1857.2004
Mongolian Agency for Standard Metrology, "MNS ISO 6579-3:2019 Food microbiology. General methods for detection, enumeration and serological differentiation of Salmonella. Part 3 /mong./
A. Abdel-Kadhim Jawad and A. H. Al-Charrakh, "Outer Membrane Protein C (ompC) Gene as the Target for Diagnosis of Salmonella Species Isolated from Human and Animal Sources."
J. Alvarez et al., "Development of a Multiplex PCR Technique for Detection and Epidemiological Typing of Salmonella in Human Clinical Samples," J Clin Microbiol, vol. 42, no. 4, pp. 1734-1738, Apr. 2004. https://doi.org/10.1128/JCM.42.4.1734-1738.2004
"EUCAST Disk Diffusion Method for Antimicrobial Susceptibility Testing Antimicrobial susceptibility testing EUCAST disk diffusion method," 2024. [Online]. Available: www.eucast.org
"Kirby-Bauer Disk Diffusion Susceptibility Test Protocol," 2009. [Online]. Available: www.atcc.org
M. P. Weinstein and Clinical and Laboratory Standards Institute, Performance standards for antimicrobial susceptibility testing.
B. V. Lubbers, C. Miller, M. G. Papich, and Clinical and Laboratory Standards Institute, Performance standards for antimicrobial disk and dilution susceptibility tests for bacteria isolated from animals.
J. Alvarez et al., "Development of a Multiplex PCR Technique for Detection and Epidemiological Typing of Salmonella in Human Clinical Samples," J Clin Microbiol, vol. 42, no. 4, pp. 1734-1738, Apr. 2004. https://doi.org/10.1128/JCM.42.4.1734-1738.2004
E. Grandolfo et al., "High mortality in foals associated with Salmonella enterica subsp. enterica Abortusequi infection in Italy," Journal of Veterinary Diagnostic Investigation, vol. 30, no. 3, pp. 483-485, May 2018. https://doi.org/10.1177/1040638717753965
B. Pakbin, W. M. Brück, and J. W. A. Rossen, "Virulence factors of enteric pathogenic Escherichia coli: A review," International Journal of Molecular Sciences, vol. 22, no. 18. Sep. 01, 2021. https://doi.org/10.3390/ijms22189922
O. G. Gó Mez-Duarte et al., "Detection of Escherichia coli Enteropathogens by Multiplex Polymerase Chain Reaction from Children's Diarrheal Stools in Two Caribbean-Colombian Cities."
A. Antonoplis, X. Zang, T. Wegner, P. A. Wender, and L. Cegelski, "Vancomycin-Arginine Conjugate Inhibits Growth of Carbapenem-Resistant E. coli and Targets Cell-Wall Synthesis," ACS Chem Biol, vol. 14, no. 9, pp. 2065-2070, Sep. 2019. https://doi.org/10.1021/acschembio.9b00565
M. Raeispour and R. Ranjbar, "Antibiotic resistance, virulence factors and genotyping of Uropathogenic Escherichia coli strains," Antimicrob Resist Infect Control, vol. 7, no. 1, Oct. 2018. https://doi.org/10.1186/s13756-018-0411-4
L. Poirel, A. Jayol, and P. Nordmanna, "Polymyxins: Antibacterial activity, susceptibility testing, and resistance mechanisms encoded by plasmids or chromosomes," Clinical Microbiology Reviews, vol. 30, no. 2. American Society for Microbiology, pp. 557-596, Apr. 01, 2017. https://doi.org/10.1128/CMR.00064-16
R. L. Skov and D. L. Monnet, "Plasmid-mediated colistin resistance (mcr-1 gene): Three months later, the story unfolds," Euro surveillance, vol. 21, no. 9. European Centre for Disease Prevention and Control (ECDC), Mar. 03, 2016. https://doi.org/10.2807/1560-7917.ES.2016.21.9.30155
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 Sarantsetseg Sarangerel, Tsegmed Baatarsuren, Nyamdavaa Guugandaa, Chimedtseren Bayasgalan, Erdene-Ochir Tseren-Ochir, Uyanga Temuujin
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright on any research article in the Mongolian Journal of Agricultural Sciences is retained by the author(s).
The authors grant the Mongolian Journal of Agricultural Sciences a license to publish the article and identify itself as the original publisher.
Articles in the Mongolian Journal of Agricultural Sciences are Open Access articles published under a Creative Commons Attribution 4.0 International License CC BY.
This license permits use, distribution and reproduction in any medium, provided the original work is properly cited.
