A Comparative study of leaves’biological activity in five different fruit species
DOI:
https://doi.org/10.5564/mjas.v15i37.3089Keywords:
DPPH, total antioxidant disease, total phenolic compounds, total flavonoidsAbstract
We collected and compared data from the leaves of five different fruit species in Mongolia—gooseberries, sea buckthorn, blackberries, and strawberries. We collected data regarding the total phenolic compounds, total flavonoids, total antioxidant capacity and DPPH free radical inhibitory activity. Extracts were taken with 50% ethanol at a ratio of 1:10 at room temperature varied in the content of biologically active substances. The content of total phenolic compounds in dry fruit leaves ranged from 34.52 mg/g (buckthorn leaves) to 55.63 mg/g (strawberry leaves) gallic acid equivalent, while the flavonoid content ranged from 16.03 mg/g (currant leaves) to 27.27 mg/g. (strawberry leaf). The total antioxidant capacity ranged from 8.21 mg/g (buckthorn leaves) to 18.4 mg/g (strawberry leaves). Meanwhile, the DPPH free radical scavenging activity ranged from 3.95 mg/g to 9.17 mg/g. Lastly, the relationship between the activity of strawberry leaves, rock leaf, blackcurrant leaf, blackberry leaf, and sea buckthorn leaf showed decreasing results (??). According to the results of the research, strawberry leaves were the most biologically active, so it is considered suitable for biologically active preparations. The biological activity of blackcurrant leaves cultivated in Mongolia was slightly lower than that of strawberry leaves, but it may be more available as a raw material.
ТАВАН ЗҮЙЛ ЖИМСНИЙ НАВЧНУУДЫН БИОЛОГИЙН ИДЭВХИЙН ХАРЬЦУУЛСАН СУДАЛГААНЫ ДҮНГЭЭС
Монгол оронд зэрлэг болон таримлаар ургадаг үхрийн нүд, чацаргана, хад, бөөрөлзгөнө, гүзээлзгэнэ зэрэг жимсний навчнууд дээр нийт фенолт нэгдэл, нийлбэр флавоноид, нийт антиоксидант багтаамж болон DPPH чөлөөт радикал дарангуйлах идэвхийг тодорхойллоо. Тасалгааны хэмд 1:10 харьцаагаар 50%-ийн этилийн спиртээр хандалсан хандны биологийн идэвхт бодисын агууламж харилцан адилгүй байв. Хуурай жимсний навчнуудын нийт фенолт нэгдлийн агууламж 34.52 мг/г (хадны навч) аас 55.63 мг/г (гүзээлзгэнэ навч) галлийн хүчлийн эквивалент-ын хооронд байсан бол флаваноидын агууламж 16,03 мг/г (үхрийн нүдний навч) аас 27.27 мг/г (гүзээлзгэнэ навч) рутины эквивалентын хооронд байв. Нийт антиоксидант багтаамжийн хувьд 8.21 мг/гр (чацарганын навч) аас 18.4 мг/г (гүзээлзгэнийн навч)-ын хооронд байсан бол DPPH чөлөөт радикал дарангуйлах идэвх 3.95 мг/г аас 9.17 мг/г -ын хооронд байсан ба идэвхийн хамаарал нь гүзээлзгэнэ навч, хадны навч, үхрийн нүдний навч, бөөрөлзгөний навч, чацарганы навч гэсэн дараалалтайгаар буурсан дүн үзүүлэв. Судалгааны ажлын дүнгээс харахад гүзээлзгэнэ жимсний навч хамгийн өндөр биологийн идэвхтэй байсан тул биологийн идэвхт бэлдмэлд тохиромжтой гэж үзэж байна. Монгол оронд тарималжуулсан үхрийн нүдний навчны биологийн идэвх гүзээлзгэнэ жимсний навчнаас бага зэрэг бага байсан боловч түүхий эдийн хувьд олдоц ихтэй байж болох юм.
Түлхүүр үг: DPPH, нийт антиоксидант багтаамж, нийт фенолт нэгдэл, нийлбэр флавоноид
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References
O. M. Mosquera, Y. M. Correa, D. C. Buitrago, and J. Niño, “Antioxidant activity of twenty five plants from Colombian biodiversity,” Mem. Inst. Oswaldo Cruz, vol. 102, no. 5, pp. 631–634, Jul. 2007, https://doi.org/10.1590/S0074-02762007005000066.
G. Bjelakovic, D. Nikolova, L. L. Gluud, R. G. Simonetti, and C. Gluud, “Mortality in Randomized Trials of Antioxidant Supplements for Primary and Secondary Prevention: Systematic Review and Meta-analysis,” JAMA, vol. 297, no. 8, p. 842, Feb. 2007, https://doi.org/10.1001/jama.297.8.842.
Y. Chu, C. Chang, and H. Hsu, “Flavonoid content of several vegetables and their antioxidant activity,” J. Sci. Food Agric., vol. 80, no. 5, pp. 561–566, Apr. 2000, https://doi.org/10.1002/(SICI)1097-0010(200004)80:5<561::AID-JSFA574>3.0.CO;2-#.
S. Y. Wang and H.-S. Lin, “Antioxidant Activity in Fruits and Leaves of Blackberry, Raspberry, and Strawberry Varies with Cultivar and Developmental Stage,” J. Agric. Food Chem., vol. 48, no. 2, pp. 140–146, Feb. 2000, https://doi.org/10.1021/jf9908345.
Q. D. Do et al., “Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica,” J. Food Drug Anal., vol. 22, no. 3, pp. 296–302, Sep. 2014, https://doi.org/10.1016/j.jfda.2013.11.001
X. Chen et al., “Optimization of extraction of phenolics from leaves of Ficus virens,” J. Zhejiang Univ. Sci. B, vol. 14, no. 10, pp. 903–915, Oct. 2013, https://doi.org/10.1631/jzus.B1200365.
E. J. Lee, N. Nomura, B. S. Patil, and K. S. Yoo, “Measurement of total phenolic content in wine using an automatic Folin-Ciocalteu assay method,” Int. J. Food Sci. Technol., vol. 49, no. 11, pp. 2364–2372, Nov. 2014, https://doi.org/10.1111/ijfs.12557.
L. Pavun, S. Uskoković-Marković, M. Jelikić-Stankov, D. Đikanović, and P. Đurđević, “Determination of flavonoids and total polyphenol contents in commercial apple juices,” Czech J. Food Sci., vol. 36, no. 3, pp. 233–238, Jun. 2018, https://doi.org/10.17221/211/2017-CJFS.
P. Prieto, M. Pineda, and M. Aguilar, “Spectrophotometric Quantitation of Antioxidant Capacity through the Formation of a Phosphomolybdenum Complex: Specific Application to the Determination of Vitamin E,” Anal. Biochem., vol. 269, no. 2, pp. 337–341, May 1999, https://doi.org/10.1006/abio.1999.4019.
Y. Hangun-Balkir and M. L. McKenney, “Determination of antioxidant activities of berries and resveratrol,” Green Chem. Lett. Rev., vol. 5, no. 2, pp. 147–153, Jun. 2012, https://doi.org/10.1080/17518253.2011.603756.
E. Enkhtuya, T. Kashiwagi, T. Shimamura, H. Ukeda, and O. Tseye-Oidov, “Screening Study on Antioxidant Activity of Plants Grown Wildly in Mongolia,” Food Sci. Technol. Res., vol. 20, no. 4, pp. 891–897, 2014, https://doi.org/10.3136/fstr.20.891.
B. Veljkovic et al., “Antioxidant and Anticancer Properties of Leaf and Fruit Extracts of the Wild Raspberry (Rubus idaeus L.),” Not. Bot. Horti Agrobot. Cluj-Napoca, vol. 47, no. 2, pp. 359–367, Dec. 2018, https://doi.org/10.15835/nbha47111274.
L. Buřičová et al., “Antioxidant capacity and antioxidants of strawberry, blackberry, and raspberry leaves,” Czech J. Food Sci., vol. 29, no. 2, pp. 181–189, Apr. 2011, https://doi.org/10.17221/300/2010-CJFS.
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