Anti-Inflammatory Screening of Ulmus pumila L ethanolic extract


  • Batmunkh Sosorburam School of Veterinary Medicine, Mongolian University of Life Sciences, Zaisan 17029, Ulaanbaatar, Mongolia
  • Dugar Delgermurun School of Veterinary Medicine, Mongolian University of Life Sciences, Zaisan 17029, Ulaanbaatar, Mongolia
  • Pelden Bolormaa School of Veterinary Medicine, Mongolian University of Life Sciences, Zaisan 17029, Ulaanbaatar, Mongolia



Dwarf elm leaves /Ulmus pumila L./, carrageenan, rat, anti-inflammatory, toxicity


Deciduous trees of Ulmus species were reported to exhibit antibiotic, antifungal, antioxidant, anti-inflammatory, hepatoprotective, neuroprotective, antiangiogenic, and antiviral effects that rich in flavonoids. In this study, phytochemical analysis and pharmacological activities of Ulmus pumila L were studied. The upper parts of Ulmus pumila L plants were grinded and extracted in 80% ethanol with a ratio of 1:10, infusing by maceration method. The total crude extract was fractioned into a group of solvents with increasing polarity: hexane, chloroform, ethyl acetate, and n-butanol. The results of the toxicity test showed the safety of the oral ingestion and treatment with ethanolic and ethyl-acetic fractions decreased inflammatory symptoms and exhibited protective effects for carrageenan-induced paw edema. These preliminary findings may support its traditional medicinal use and could be promising candidate with application in the treatment of inflammatory symptoms.


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M. Farouk R., E. Z. Soheir M., G. Neveen S., S. Omar M., F. Walid, and B. Ann G., “Phytoconstituents with cytotoxic activity from Ulmus pumila L.,” J. Appl. Pharm. Sci., May 2021,

S. Baasanmunkh et al., “Flora of Mongolia: annotated checklist of native vascular plants,” PhytoKeys, vol. 192, pp. 63–169, Mar. 2022,

“Chemotherapeutic effect of Ulmus pumila leaves methanolic extract against N-methyl-N-nitrosourea-induced mammary carcinoma in female rats: An in vitro and in vivo study,” J. Appl. Pharm. Sci., vol. 9, no. 12, pp. 57–68, Dec. 2019,

Nyamosor Batkhuu, Tsedensodnom Enkhchimeg, and Tsogtbaatar Jamsran, “Growth characteristic of Ulmus pumila L. seedlings from different seed sources in Mongolia.,” 2014,

C. Ghosh, S. H. Yang, and S. G. Hwang, “Methanol extract of Ulmus pumila. L exerts potent anti‐inflammatory effects in murine macrophages and mouse skin,” FASEB J., vol. 27, no. S1, Apr. 2013,

H.-J. Jung et al., “Anti-angiogenic activity of the methanol extract and its fractions of Ulmus davidiana var. japonica,” J. Ethnopharmacol., vol. 112, no. 2, pp. 406–409, Jun. 2007,

Zhang, Zhang, Li, Wang, Qu, and Fan, “Transcriptome Analysis of Elm (Ulmus pumila) Fruit to Identify Phytonutrients Associated Genes and Pathways,” Forests, vol. 10, no. 9, p. 738, Aug. 2019,

Wagner H, Bladt S., Plant Drug Analysis. Berlin: Springer-Verlag; 1996.

Ž. Maleš, M. Plazibat, V. Vundać, I. Žuntar, and K. Pilepić, “Thin-layer chromatographic analysis of flavonoids, phenolic acids, and amino acids in some Croatian Hypericum taxa,” J. Planar Chromatogr. – Mod. TLC, vol. 17, no. 4, pp. 280–285, Aug. 2004,

B. Kaya, Y. Menemen, and F. Saltan, “Flavonoid compounds identified in Alchemilla l. species collected in the North-eastern Black sea region of Turkey,” Afr. J. Tradit. Complement. Altern. Med., vol. 9, no. 3, pp. 418–425, Sep. 2012,

C. J. Morris, “Carrageenan-Induced Paw Edema in the Rat and Mouse,” in Inflammation Protocols, vol. 225, New Jersey: Humana Press, 2003, pp. 115–122.

C. A. Winter, E. A. Risley, and G. W. Nuss, “Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Antiinflammatory Drugs,” Exp. Biol. Med., vol. 111, no. 3, pp. 544–547, Dec. 1962,

V. B. Prozorovskiĭ and M. P. Prozorovskaia, “[Table method of determining the ED50 (LD50) of substances with low biological activity],” Farmakol. Toksikol., vol. 43, no. 6, pp. 733–735, 1980.

I. V. Berezovskaya, “Classification of Substances with Respect to Acute Toxicity for Parenteral Administration,” Pharm. Chem. J., vol. 37, no. 3, pp. 139–141, 2003,

H. Boudaoud-Ouahmed, S.-A. Tiab, N. Saidani, M. Gherrou, K. Ziane, and D. Atmani, “Phytochemical screening and pharmacological activities of Ulmus campestris bark extracts,” Orient. Pharm. Exp. Med., vol. 15, no. 4, pp. 353–363, Dec. 2015,

L. Rahmawati et al., “Anti-Inflammatory Activities of the Ethanol Extract of Prasiola japonica, an Edible Freshwater Green Algae, and Its Various Solvent Fractions in LPS-Induced Macrophages and Carrageenan-Induced Paw Edema via the AP-1 Pathway,” Molecules, vol. 27, no. 1, p. 194, Dec. 2021,




How to Cite

Sosorburam, B., Delgermurun, D., & Bolormaa, P. (2022). Anti-Inflammatory Screening of Ulmus pumila L ethanolic extract. Mongolian Journal of Agricultural Sciences, 15(36), 21–26.